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Related Experiment Videos

Staphylococcus aureus accessory regulators: expression within biofilms and effect on adhesion.

J Pratten1, S J Foster, P F Chan

  • 1Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK. jpratten@eastman.ucl.ac.uk

Microbes and Infection
|July 11, 2001
PubMed
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The accessory gene regulator (agr) and staphylococcal accessory regulator (sar) influence Staphylococcus aureus attachment to surfaces. These regulators show higher expression in deeper biofilm layers, impacting bacterial adhesion.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Biofilm Formation

Background:

  • Virulence factor expression in Staphylococcus aureus is primarily regulated by the accessory gene regulator (agr) and staphylococcal accessory regulator (sar).
  • Bacteria in natural environments predominantly exist in surface-attached biofilms, a lifestyle that can influence gene regulation.
  • Understanding agr and sar roles in biofilm formation and surface attachment is crucial for controlling S. aureus infections.

Purpose of the Study:

  • To investigate the influence of agr and sar on Staphylococcus aureus attachment to abiotic surfaces.
  • To determine the expression levels of agr and sar operons within developing biofilms.
  • To elucidate the pleiotropic effects of agr and sar on bacterial adhesion to different substrata.

Main Methods:

Related Experiment Videos

  • Utilized S. aureus strains with targeted mutations in agr and sar.
  • Employed reporter constructs to monitor agr and sar operon expression in biofilms.
  • Assessed bacterial adherence to glass and fibronectin-coated glass surfaces.

Main Results:

  • The sarA mutant strain exhibited enhanced adherence to glass compared to wild-type and agrA mutant strains.
  • All tested strains showed increased adherence to fibronectin-coated surfaces.
  • agr and sar expression was significantly elevated in the deeper layers of biofilms, correlating with bacterial density.

Conclusions:

  • agr and sar play significant roles in modulating Staphylococcus aureus surface attachment and biofilm development.
  • These regulators demonstrate pleiotropic effects on the expression of adhesion molecules, influencing binding to various surfaces.
  • Cell density-dependent regulation of agr and sar is evident within the complex biofilm environment.