Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses
Summary
This summary is machine-generated.Carbohydrates on Japanese cedar pollen allergen Cry j 1 are crucial for driving T helper 2 (T(H)2) responses. Destroying these allergen carbohydrates significantly reduced T(H)2 cell proliferation and cytokine production in vitro.
Area Of Science
- Allergen immunotherapy
- Immunology
- Molecular biology
Background
- Allergen carbohydrates are important for allergenicity.
- The role of carbohydrates in driving T(H)2 responses remains unclear.
Purpose Of The Study
- To investigate the role of carbohydrates on Cry j 1 in T(H)2 responses.
- Cry j 1 is the major allergen in Japanese cedar pollen.
Main Methods
- Carbohydrates on Cry j 1 were destroyed using periodate-oxidation.
- Cellular responses (proliferation, cytokine production) were compared between native, treated, and mock-treated Cry j 1.
- Peripheral blood mononuclear cells, T-cell lines, and clones from patients with Japanese cedar pollinosis were used.
Main Results
- Periodate-treated Cry j 1 significantly decreased proliferation and IL-5 production in peripheral blood mononuclear cells.
- T-cell clones showed reduced proliferation and IL-4/IL-5 production, but not IFN-gamma, against treated Cry j 1.
- Mannose receptor blockade did not affect cellular responses.
Conclusions
- Carbohydrates on Cry j 1 play a significant role in promoting T(H)2 responses in vitro.
- These carbohydrates are not major T-cell epitopes.
View abstract on PubMed