Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apolipoprotein E and beta A4-amyloid: signals and effects.

T G Ohm1, U Hamker, A Cedazo-Minguez

  • 1Institute fur Anatomie, University Klinikum Charité, D-10098 Berlin, Germany.

Biochemical Society Symposium
|July 13, 2001
PubMed
Summary

The apolipoprotein E4 (APOE epsilon 4) allele accelerates Alzheimer's disease onset by increasing intraneuronal calcium levels. This effect is linked to cholesterol transport and tau pathology, highlighting APOE as a key Alzheimer's risk factor.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Lipoprotein (a) on the Quantification of LDL-Cholesterol.

Clinical laboratory·2026
Same author

[Primary disorders of lipid metabolism: their place in current dyslipidemia guidelines and treatment innovations].

Innere Medizin (Heidelberg, Germany)·2023
Same author

NT-proBNP and stem cell factor plasma concentrations are independently associated with cardiovascular outcomes in end-stage renal disease hemodialysis patients.

European heart journal open·2023
Same author

NT-proBNP for risk prediction of cardiovascular events and all-cause mortality: The getABI-study.

International journal of cardiology. Heart & vasculature·2020
Same author

Current and emerging avenues for Alzheimer's disease drug targets.

Journal of internal medicine·2019
Same author

Acute Exercise-Induced Circulating Haematopoietic Stem and Progenitor Cells in Cardiac Patients - A Case Series.

Heart, lung & circulation·2018

Area of Science:

  • Neuroscience
  • Genetics
  • Biochemistry

Background:

  • The apolipoprotein E (APOE) gene has three common alleles: APOE epsilon 2, 3, and 4.
  • APOE epsilon 4 is the primary genetic risk factor for sporadic Alzheimer's disease (AD), independent of age.
  • Individuals with APOE epsilon 4 experience earlier onset of tau pathology compared to APOE epsilon 3 homozygotes.

Purpose of the Study:

  • To investigate the role of apolipoprotein E (ApoE) isoforms in Alzheimer's disease (AD) pathogenesis.
  • To elucidate the mechanism by which ApoE influences intraneuronal calcium levels and its interaction with beta-amyloid.
  • To explore the relationship between ApoE, cholesterol metabolism, and tau pathology in AD.

Main Methods:

  • Examined the dose-dependent effects of ApoE isoforms (E4, E3, E2) on intraneuronal calcium levels.

Related Experiment Videos

  • Investigated the influence of beta A4-peptide on ApoE's calcium-elevating effects.
  • Assessed the impact of ApoE on cholesterol transport and tau phosphorylation in neurons.

    • Main Results:

    • ApoE dose-dependently increases intraneuronal calcium, with the order ApoE4 > ApoE3 > ApoE2.
    • Beta A4-peptide amplifies ApoE's calcium-elevating effect, mediated by P/Q-type-like calcium channels.
    • Astrocytic ApoE production decreases in the presence of beta A4-peptide, reducing ApoE bioavailability.
    • ApoE influences neuronal cholesterol levels, which in turn affects tau phosphorylation at AD-relevant sites.

    Conclusions:

    • The APOE epsilon 4 allele significantly contributes to AD risk by modulating intraneuronal calcium homeostasis.
    • ApoE's interaction with beta-amyloid and its influence on cholesterol metabolism are critical pathways in AD pathogenesis.
    • Targeting ApoE pathways may offer therapeutic strategies for Alzheimer's disease.