Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Postbinding fusion function contributed by a chimeric murine leukemia virus envelope protein.

H Nakamura1, A Takeda, T Matano

  • 1AIDS Research Center, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.

Archives of Virology
|July 13, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response of feline intradental nerve fibers to tooth cutting by Er:YAG laser.

Lasers in surgery and medicine·2000
Same author

[Care of diabetic patients. 2. The importance of patient education].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·2000
Same author

Clinical benefits of steroid therapy on surgical stress in patients with esophageal cancer.

Surgery·2000
Same author

Serum p53 antibody is a useful tumor marker in superficial esophageal squamous cell carcinoma.

Cancer·2000
Same author

Impact of circulating p53 autoantibody monitoring after endoscopic resection in mucosal gastric cancer.

Endoscopy·2000
Same author

DNA single-strand breaks are increased in muscle diseases with rimmed vacuoles.

Acta neuropathologica·2000

This study investigated a chimeric Friend murine leukemia virus (FMLV) envelope protein (Env) with human CD4. Results show this CD4-Env chimera retains post-binding fusion capabilities, crucial for viral entry.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Friend murine leukemia virus (FMLV) envelope protein (Env) mediates viral entry.
  • A chimeric FMLV Env was engineered, replacing the receptor-binding domain (RBD) with human CD4.
  • The functional integrity of the post-binding fusion activity of this CD4-Env chimera requires investigation.

Purpose of the Study:

  • To determine if the CD4-Env chimera retains its post-binding fusion function.
  • To assess the role of the CD4-Env chimera in viral-mediated membrane fusion.
  • To understand the contribution of the CD4-Env chimera in conjunction with FMLV receptor interactions.

Main Methods:

  • Construction of pseudotype murine leukemia viruses (MLVs) bearing the CD4-Env chimera.

Related Experiment Videos

  • Infectivity assays using NIH 3T3 cells and HeLa cells (with and without FMLV receptor, mCAT1).
  • Co-expression studies of CD4-Env with fusion-deficient FMLV Env to induce syncytia formation.

    • Main Results:

    • Pseudotype MLV with CD4-Env alone showed no infectivity.
    • Infectivity was observed when CD4-Env was co-expressed with a fusion-deficient FMLV Env in NIH 3T3 cells.
    • Syncytia formation occurred in mCAT1-expressing cells, indicating CD4-Env's role in FMLV-mediated fusion.

    Conclusions:

    • The CD4-Env chimera possesses intact post-binding fusion function.
    • The CD4-Env chimera can contribute to membrane fusion in the context of FMLV RBD-mCAT1 interactions.
    • This chimeric Env system provides insights into viral fusion mechanisms and receptor engagement.