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Updated: Aug 8, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Published on: November 26, 2015
Integrin-linked kinase expression increases with prostate tumor grade
J R Graff1, J A Deddens, B W Konicek
1Cancer Division, Lilly Research Labs, Eli Lilly and Company, Indianapolis, Indiana 46285, USA. graff_jeremy@lilly.com
Integrin-linked kinase (ILK) expression significantly increases with prostate cancer progression and is linked to higher cell proliferation and poorer patient survival, implicating ILK in tumor advancement.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Integrin-linked kinase (ILK) overexpression is implicated in tumor progression, anoikis suppression, and invasion.
- ILK inhibition in prostate adenocarcinoma (CaP) cells induces cell cycle arrest and apoptosis.
- ILK expression escalates with androgen-independent CaP progression.
Purpose of the Study:
- To investigate the correlation between ILK expression and the development and progression of prostate cancer.
- To determine if ILK levels are associated with CaP aggressiveness and patient outcomes.
Main Methods:
- Immunohistochemistry was employed to evaluate ILK expression in 100 human prostate tissue samples.
- Analysis included comparisons between high-grade CaP, low-grade CaP, and benign prostatic hyperplasia.
Main Results:
- ILK expression significantly increases with CaP progression, being higher in high-grade CaP than benign tissues.
- Elevated ILK immunostaining correlates with increased proliferative index in CaP.
- Intense ILK expression is inversely associated with 5-year patient survival.
Conclusions:
- ILK expression dramatically rises with CaP progression.
- Increased ILK is linked to the elevated proliferation driving CaP cell gain.
- Enhanced ILK expression serves as a negative prognostic indicator for 5-year survival in prostate cancer patients.
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