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Effect of melatonin administration on activities of some lysosomal enzymes in the mouse
B Witek1, E Ochwanowska, A Kolataj
1Department of Genetics, Institute of Biology, Swietokrzyska Academy, Poland.
Objectives:
Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated.
Methods:
The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes.
Results:
Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney.
Conclusion:
Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups.