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Related Experiment Videos

[Lipids from the green algae Botryococcus during staged growth in batch mode].

G S Kalacheva1, N O Zhila, T G Volova

  • 1Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia.

Mikrobiologiia
|July 14, 2001
PubMed
Summary

This study details the lipid composition of Botryococcus green algae, finding significant changes in triacylglycerides during senescence. Hydrocarbon content remained low and stable across culture ages.

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Area of Science:

  • Algal biotechnology
  • Lipidomics
  • Biochemistry

Context:

  • Botryococcus algae are a promising source of lipids.
  • Understanding lipid dynamics is crucial for optimizing algal cultivation.
  • Batch culture conditions were employed to study lipid profiles.

Purpose:

  • To characterize the lipid profile of Botryococcus green algae.
  • To investigate changes in lipid composition during different growth phases, particularly senescence.
  • To analyze the fatty acid composition of intracellular lipids.

Summary:

  • The lipid fraction of Botryococcus algae primarily consists of polar lipids, diacylglycerides, triacylglycerides, steroids, free fatty acids, and hydrocarbons.
  • Senescent cultures showed a decrease in polar lipids and a significant increase (over fourfold) in triacylglycerides.

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  • Hydrocarbon content was consistently low (≤0.9%) and independent of culture age.
  • Intracellular lipids contained a mix of saturated and unsaturated fatty acids, with C16:3 and alpha-C18:3 peaking at 35% during active growth.
  • Extracellular and intracellular lipid profiles exhibited distinct differences in lipid proportions and fatty acid patterns.

    • Impact:

    • Provides detailed insights into Botryococcus lipid metabolism.
    • Informs strategies for enhancing lipid production in algae.
    • Contributes to the understanding of algal biomass composition for potential applications.