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Hydrodynamic damage to animal cells.

¹Institute of Technology and Engineering, Massey University, Palmerston North, New Zealand. Y.Chisti@massey.ac.nz

Critical Reviews in Biotechnology

|July 14, 2001

Summary

Hydrodynamic forces in bioprocessing impact animal cells, causing biochemical and physiological responses like apoptosis. This review quantizes these forces and cell response thresholds.

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Area of Science:

Biotechnology
Cell Biology
Bioprocessing Engineering

Background:

Animal cells in bioprocessing are exposed to hydrodynamic forces during culture, transfer, and recovery.
These forces can originate from various equipment like culture vessels and microfiltration systems.
Cellular responses to hydrodynamic forces vary based on force characteristics and cell type.

Purpose of the Study:

To review hydrodynamic forces encountered in bioprocessing equipment.
To analyze the impact of these forces on animal cells.
To provide methods for quantifying forces and identifying cell response thresholds.

Main Methods:

Literature review of hydrodynamic forces in bioprocessing.
Analysis of reported cellular responses (biochemical and physiological).
Examination of methods for force quantification and response threshold determination.

Main Results:

Hydrodynamic forces can induce apoptosis and mechanical destruction in animal cells.
Cellular responses are dependent on force type, intensity, duration, and cell characteristics.
Specific methods for quantifying forces and thresholds for different cell culture types are presented.

Conclusions:

Understanding hydrodynamic forces is critical for optimizing cell culture and bioprocessing.
Quantifying forces and response thresholds helps mitigate adverse cellular effects.
This review provides a framework for assessing hydrodynamic stress in bioprocessing environments.

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