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Nonenzymatic chloramphenicol resistance mediated by IncC plasmid R55 is encoded by a floR gene variant
A Cloeckaert1, S Baucheron, E Chaslus-Dancla
1Station de Pathologie Aviaire et Parasitologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France. cloeckae@tours.inra.fr
Abstract:
The IncC plasmid R55, initially described in the 1970s and isolated from Klebsiella pneumoniae, confers nonenzymatic chloramphenicol resistance. The gene coding for this resistance was cloned and sequenced and shows 95 to 97% nucleotide identity with the recently reported floR gene from Salmonella enterica serovar Typhimurium DT104 and from Escherichia coli animal isolates, respectively, conferring cross-resistance to florfenicol.
Insights
The IncC plasmid R55 provides chloramphenicol resistance in Klebsiella pneumoniae. Its resistance gene is highly similar to the floR gene, indicating cross-resistance to florfenicol.
Area of Science:
- Microbiology
- Genetics
- Antimicrobial Resistance
Background:
- The IncC plasmid R55, identified in the 1970s, confers chloramphenicol resistance in Klebsiella pneumoniae.
- Chloramphenicol resistance is a significant concern in clinical settings.
Purpose of the Study:
- To characterize the gene responsible for chloramphenicol resistance on the IncC plasmid R55.
- To investigate the relationship between this resistance gene and other known resistance mechanisms.
Main Methods:
- Cloning and sequencing of the resistance gene from IncC plasmid R55.
- Nucleotide sequence comparison with known antibiotic resistance genes.
Main Results:
- The gene conferring chloramphenicol resistance on R55 was successfully cloned and sequenced.
- The identified gene exhibits 95-97% nucleotide identity to the floR gene.
- This high similarity suggests cross-resistance to florfenicol.
Conclusions:
- The IncC plasmid R55 carries a chloramphenicol resistance gene homologous to floR.
- This finding highlights potential cross-resistance to florfenicol mediated by this plasmid-borne gene.