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Influence of liposomal amphotericin B on CD8 T-cell function
M Kretschmar1, G Geginat, T Bertsch
1Institut für Medizinische Mikrobiologie und Hygiene, Fakultät für Klinische Medizin Mannheim der Universität Heidelberg, 68167 Mannheim, Germany.
Antimicrobial Agents and Chemotherapy
|July 14, 2001
Summary
Liposomal amphotericin B showed immunosuppressive effects on immune cells in mice. Therapeutic doses in humans were found to be immunosuppressive, impacting cell lysis and T cell protection against Listeria.
Area of Science:
- Immunology
- Pharmacology
- Infectious Diseases
Background:
- Liposomal amphotericin B is an antifungal medication.
- Its impact on the immune system requires further investigation, particularly in vivo.
- Cytotoxic CD8 T cells play a crucial role in host defense against Listeria.
Purpose of the Study:
- To evaluate the immunosuppressive effects of liposomal amphotericin B.
- To compare its effects with standard amphotericin B in a murine model.
- To assess the impact on cell-mediated immunity during Listeria infection.
Main Methods:
- In vitro assays for target cell lysis.
- In vivo studies using a murine model of Listeria infection.
- Assessment of cytotoxic CD8 T cell activity.
Main Results:
- Liposomal amphotericin B demonstrated immunosuppression in vitro.
- In vivo, a dose of 5 mg/kg/day showed immunosuppressive effects comparable to standard amphotericin B at 1 mg/kg/day.
- A lower dose of 1 mg/kg/day of liposomal amphotericin B was not immunosuppressive in vivo.
Conclusions:
- Liposomal amphotericin B exhibits immunosuppressive properties.
- Dosage is critical in determining the in vivo immunosuppressive potential.
- Further research is needed to understand the clinical implications of these findings in antifungal therapy.