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Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia
I Madinier1, T Fosse, J Giudicelli
1Laboratoire de Pathobiologie Orale, Faculté de Chirurgie Dentaire, 06357 Nice Cedex 4, France.
Antimicrobial Agents and Chemotherapy
|July 14, 2001
Abstract:
The gene encoding a beta-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a beta-lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e beta-lactamases.