Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tubulin sorting during dimerization in vivo.

H D Hoyle1, F R Turner, L Brunick

  • 1Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, Indiana 47405, USA. hhoyle@bio.indiana.edu

Molecular Biology of the Cell
|July 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental taphonomy of giant sulphur bacteria: implications for the interpretation of the embryo-like Ediacaran Doushantuo fossils.

Proceedings. Biological sciences·2011
Same author

A light and electron microscopical study of the spermathecae and ventral receptacle of Anastrepha suspensa (Diptera: Tephritidae) and implications in female influence of sperm storage.

Arthropod structure & development·2007
Same author

Axoneme-specific beta-tubulin specialization: a conserved C-terminal motif specifies the central pair.

Current biology : CB·2001
Same author

Embryonic expression of the divergent Drosophila beta3-tubulin isoform is required for larval behavior.

Genetics·2001
Same author

Dissociability, modularity, evolvability.

Evolution & development·2001
Same author

Novel gene expression patterns in hybrid embryos between species with different modes of development.

Evolution & development·2001
Same journal

Mechanisms underpinning chromosome structure in metazoans.

Molecular biology of the cell·2026
Same journal

Conserved and Divergent Modes of Substrate Interaction Define Selective Localizations and Functions of a Cdc14 Phosphatase.

Molecular biology of the cell·2026
Same journal

Dimerization of the centriolin-like protein Nud1 governs spindle pole body inheritance in budding yeast.

Molecular biology of the cell·2026
Same journal

Non-muscle Myosin II acts as a negative feedback mediator to control cell contraction dynamics in adherent cells.

Molecular biology of the cell·2026
Same journal

The tetraspanin disc proteins, peripherin-2 and ROM1, facilitate CNG channel localization to the rod outer segment.

Molecular biology of the cell·2026
Same journal

Csf1 facilitates adaptive membrane lipid remodeling linked to ER-plasma membrane contact sites.

Molecular biology of the cell·2026
See all related articles

Beta-tubulin isoforms sort during dimerization, with the carboxyl terminus crucial for stable alpha-beta heterodimer formation. This sorting impacts microtubule function and axoneme structure in Drosophila.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • Microtubules are essential cytoskeletal components formed by alpha-beta tubulin heterodimers.
  • Different beta-tubulin isoforms exist, but their specific roles in heterodimerization and microtubule assembly are not fully understood.
  • The carboxyl terminus of beta-tubulin is a variable region with unknown structural and functional significance.

Purpose of the Study:

  • To investigate the sorting of beta-tubulin isoforms during heterodimerization in the Drosophila male germ line.
  • To determine the role of the beta-tubulin carboxyl terminus in alpha-beta heterodimer formation and stability.
  • To elucidate how differential beta-tubulin incorporation affects microtubule function and axoneme assembly.

Main Methods:

Related Experiment Videos

  • Studied beta-tubulin isoform dimerization with alpha-tubulin under varying alpha-tubulin availability.
  • Utilized a beta-tubulin mutant lacking the carboxyl terminus (beta 2 Delta C) to assess its dimerization and incorporation properties.
  • Analyzed microtubule assembly and axoneme structure in Drosophila, including motility and periodicity, following incorporation of different dimer types.

    • Main Results:

    • Distinct beta-tubulin isoforms show differential affinities for alpha-tubulin during dimerization.
    • The beta-tubulin carboxyl terminus is critical for forming stable alpha-beta heterodimers, especially when alpha-tubulin is limiting.
    • Beta 2 Delta C dimers incorporate into axonemes but lead to nonmotile structures due to disrupted periodicity of associated proteins.

    Conclusions:

    • Beta-tubulin sorting during dimerization is a key mechanism for regulating isoform-specific microtubule functions.
    • The carboxyl terminus of beta-tubulin plays a dual role: facilitating heterodimerization and mediating interactions with other axonemal components.
    • Differential use of beta-tubulin isoforms and their structural features are essential for building functional microtubule-based structures like axonemes.