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Updated: Oct 5, 2026
Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
Published on: March 30, 2016
Isolation and characterization of human foetal alpha-globulin (alpha 1F) from foetal and hepatoma sera
A Adinolfi1, M Adinolfi, S Cohen
1Paediatric Research Unit, Guy's Hospital Medical School, London, S.E. 1., Great Britain.
Abstract:
The alpha 1F present in human foetal sera and the serum from a patient with hepatocellular carcinoma was isolated by immune precipitation using rabbit anti-alpha 1F. After labelling with 125I, some physicochemical properties of alpha 1F were investigated. The molecular weights of 125I-labelled alpha 1F isolated from foetal and hepatoma sera were 61,000 and 63,000, respectively. The protein was not dissociated in 6 M guanidine after complete reduction, showing that it contains a single peptide chain.
