[Measurement of biochemical components in individuals with infrared spectroscopy]

We have developed the Fourier transform infrared spectral analysis system to measure constituents of human oral mucosa in vivo and clinically obtained serum for non-invasive diagnosis. The attenuated total reflectance(ATR) probe in rod shape was used for the measurements of both oral tissue and serum. The results indicate that the change of in vivo oral mucosal infrared spectra correlated with that of blood triglyceride level, and rapidly analyzed by artificial neural networks successfully. The serum glucose level was correlated with glycan-related infrared bands, although the correlation coefficient(r2) was not high enough(0.58). When more sophisticated analytical methods are developed for multiple infrared data sets using a miniaturized instrument, the application of Fourier transform infrared analytical technique would be more advanced in the field of non-invasive clinical diagnosis for individuals.