Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunochemical demonstration of plasminogen phenotypes using electroblotting.

C Pytlik1, S Rand, B Brinkmann

  • 1Institut für Rechtsmedizin der Universität Münster, Federal Republic of Germany.

International Journal of Legal Medicine
|December 1, 1990
PubMed
Summary

This study analyzed plasminogen (PLG) polymorphism in 500 individuals, identifying 8 phenotypes and confirming autosomal codominant inheritance. PLG phenotypes are detectable in aged bloodstains and diluted sera.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DVD Versus Physiotherapist-Led Inhaler Education: A Randomised Controlled Trial.

Irish medical journal·2018
Same author

[Patio repair for urethrocutaneous fistulae : Results of a multicentre retrospective study].

Der Urologe. Ausg. A·2017
Same author

The Necessity of Developing New Criteria for Carcinogen Classification of Fibers by the Mak Commission.

Inhalation toxicology·2015
Same author

Physiotherapy in cystic fibrosis: optimising techniques to improve outcomes.

Paediatric respiratory reviews·2013
Same author

High-gain nondegenerate two-wave mixing in Cr:YAIO(3).

Optics letters·2009
Same author

Fatal mycotic aneurysm caused by Pseudallescheria boydii after near drowning.

International journal of legal medicine·2009

Area of Science:

  • Genetics
  • Biochemistry

Background:

  • Plasminogen (PLG) is crucial for fibrinolysis.
  • Understanding PLG genetic variation is important for various applications.

Purpose of the Study:

  • To analyze the polymorphism of plasminogen (PLG).
  • To determine allele frequencies and inheritance patterns.
  • To assess the stability and detectability of PLG phenotypes.

Main Methods:

  • Isoelectric focusing on polyacrylamide gels followed by electroblotting.
  • Analysis of neuraminidase-pretreated sera from 500 unrelated individuals.
  • Family data analysis from 300 mother-child pairs.

Main Results:

  • Eight PLG phenotypes were observed in the study population.

Related Experiment Videos

  • Allele frequencies were determined: PLG*1 (0.708), PLG*2 (0.274), PLG*3 (0.013), PLG*V (0.005).
  • Autosomal codominant inheritance was confirmed; phenotypes were detectable in aged bloodstains and diluted sera (up to 1:6 for PLG phenotype 1).

    • Conclusions:

    • The study established the genetic basis of plasminogen polymorphism.
    • PLG phenotyping is feasible in various biological samples and conditions.
    • A theoretical exclusion rate of 22.6% was calculated for paternity testing or forensic applications.