Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide and bone.

R J van't Hof1, S H Ralston

  • 1Department of Medicine and Therapeutics, University of Aberdeen, Aberdeen, UK. r.hof@abdn.ac.uk

Immunology
|July 17, 2001
PubMed
Summary

Nitric oxide (NO) impacts bone health. While high levels from inflammation can harm bone, NO produced by endothelial nitric oxide synthase (eNOS) is vital for bone formation and turnover.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effect of Anti-rheumatic Drugs on the Skeleton.

Calcified tissue international·2022
Same author

Risk of severe COVID-19 in patients with inflammatory rheumatic diseases treated with immunosuppressive therapy in Scotland.

Scandinavian journal of rheumatology·2022
Same author

Diagnosis and Management of Osteoporosis During COVID-19: Systematic Review and Practical Guidance.

Calcified tissue international·2021
Same author

Rheumatology in a time of Coronavirus: lessons from our early experiences.

QJM : monthly journal of the Association of Physicians·2020
Same author

Cognitive function, disease burden and the structural connectome in systemic lupus erythematosus.

Lupus·2018
Same author

High mortality in younger patients with major osteoporotic fractures.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016

Area of Science:

  • Bone Biology
  • Cell Signaling
  • Inflammation

Background:

  • Nitric oxide (NO) is a free radical influencing bone cell function.
  • Endothelial NOS (eNOS) is constitutively expressed in bone, while inducible NOS (iNOS) is activated by inflammatory stimuli.
  • The role of neuronal NOS in bone cells is not fully understood.

Purpose of the Study:

  • To explore the multifaceted roles of nitric oxide (NO) and its synthase isoforms in bone metabolism.
  • To investigate the impact of NO on bone formation, resorption, and its relevance in inflammatory bone diseases like rheumatoid arthritis.
  • To assess the therapeutic potential of the L-arginine/NO pathway for bone disease treatment.

Main Methods:

  • Review of existing literature on nitric oxide synthase (NOS) expression and function in bone cells.
  • Analysis of studies involving NOS knockout models and the effects of pro-inflammatory cytokines (IL-1, TNF, Interferon gamma).
  • Examination of research on the influence of estrogen, mechanical loading, and pharmacological NO donors on bone.

Main Results:

  • Pro-inflammatory cytokines activate iNOS, leading to NO production that exacerbates cytokine-induced bone loss, relevant to osteoporosis in inflammatory conditions.
  • High NO levels, particularly from iNOS activation by cytokines like Interferon gamma, can inhibit bone resorption and formation, potentially suppressing turnover in severe inflammation.
  • eNOS plays a crucial role in regulating osteoblast activity and bone formation, mediating effects of estrogen and mechanical loading, and its deficiency leads to osteoporosis.
  • Pharmacological NO donors show promise in increasing bone mass in animal models and may influence human bone turnover.

Conclusions:

  • The L-arginine/NO pathway is implicated in both detrimental (iNOS) and beneficial (eNOS) effects on bone metabolism.
  • eNOS-derived NO is critical for normal bone formation, mediating hormonal and mechanical influences.
  • Targeting the L-arginine/NO pathway offers a novel therapeutic strategy for preventing and treating bone diseases, including osteoporosis.

Related Experiment Videos