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Clonal analysis of B cells in the osteoarthritis synovium
S Shiokawa1, N Matsumoto, J Nishimura
1Department of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Tsurumihara 4546, Beppu, Oita 874-0838, Japan. sahasu@tsurumi.beppu.kyushu-u.ac.jp
Objectives:
Cellular and humoral immunity to collagen and cartilage proteoglycan were shown in patients with osteoarthritis (OA). Inflammatory infiltration containing T and B lymphocytes and macrophages, which are HLA-DR positive, is often seen in the synovial membrane of patients with OA. An analysis of the DNA restriction enzyme patterns of T lymphocytes from the OA synovium showed an oligoclonal pattern of T cell receptor beta chain gene rearrangements. No similar studies of B cell clonality have previously been performed. This study aimed at determining the clonal characteristics of the B cells in the OA synovium.
Methods:
A reverse transcriptase-polymerase chain reaction of the immunoglobulin transcripts of B cells in the synovial membranes from six patients with OA was performed and the products were analysed by a single strand conformation polymorphism analysis.
Results:
Several dominant bands were seen in all samples and some of the dominant bands were common among the two or three separate regions of each synovial sample.
Conclusion:
Infiltrated B cells are oligoclonal, and an antigen driven immune response may play a part in the progression of the disease process in OA.