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Related Experiment Videos

Neutralized lanthanum solution: a largely noncolloidal ultrastructural tracer.

P F Schatzki, A Newsome

    Stain Technology
    |May 1, 1975
    PubMed
    Summary

    Lanthanum nitrate solution primarily exists as charged particles under pH 7.7, with 80% being ultrafiltrable. This suggests lanthanum

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    Area of Science:

    • Analytical Chemistry
    • Materials Science
    • Biochemistry

    Background:

    • Lanthanum compounds are utilized in various scientific applications.
    • Understanding the physicochemical properties of lanthanum solutions is crucial for its effective use.

    Purpose of the Study:

    • To characterize the size and charge of lanthanum particles in solution.
    • To investigate the influence of pH on lanthanum speciation.
    • To correlate these properties with lanthanum's utility in electron microscopy.

    Main Methods:

    • Column chromatography with an 1800 dalton exclusion limit.
    • Ultrafiltration using a 500 dalton membrane.
    • Conductivity measurements.
    • Colorimetric analysis for lanthanum concentration.

    Main Results:

    • Lanthanum was not excluded by the 1800 dalton column, indicating smaller particles.
    • Approximately 75% of lanthanum passed through the 500 dalton ultrafilter.
    • Conductivity decreased by 20% up to pH 7.7, suggesting charge reduction.
    • At pH 7.7, 80% of lanthanum existed as charged particles <500 daltons.

    Conclusions:

    • Lanthanum exists predominantly as small, charged particles at physiological pH.
    • A smaller fraction forms larger, potentially colloidal particles.
    • Electron microscopy staining effectiveness correlates with the ultrafiltrable, non-colloidal lanthanum ion.

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