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Testis-specific expression of an intronless gene encoding a human poly(A) polymerase.

Y J Le1, H Kim, J H Chung

  • 1Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Taejon. ylee@mail.kaist.ac.kr

Molecules and Cells
|July 19, 2001
PubMed
Summary

Researchers identified a human testis-specific poly(A) polymerase (hPAPT) gene, similar to its mouse counterpart (mPAPT). These intronless genes likely arose via retrotransposition, suggesting conserved roles in mammalian spermatogenesis.

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Reproductive Biology

Background:

  • A mouse testis-specific poly(A) polymerase (mPAPT) gene was identified, potentially regulating polyadenylation during spermatogenesis.
  • Understanding the evolutionary conservation of PAPT genes across mammals is crucial for elucidating their function.

Purpose of the Study:

  • To isolate and characterize the human homolog of mPAPT (hPAPT) to investigate its conservation in mammals.
  • To analyze the structural and genomic similarities and differences between human and mouse PAPT proteins and genes.

Main Methods:

  • Isolation of human cDNA encoding hPAPT.
  • Structural comparison of hPAPT and mPAPT.
  • Genomic DNA analysis of the hPAPT gene.
  • Phylogenetic analysis of PAPT gene evolution.

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Main Results:

  • A human cDNA homolog, hPAPT, specifically expressed in the testis, was isolated.
  • hPAPT shares structural similarities with mPAPT, notably lacking C-terminal residues found in PAP II.
  • The hPAPT gene is intronless, mirroring the mPAPT gene structure.
  • Sequence homology between hPAPT and mPAPT is lower than between their respective PAP II counterparts.
  • Phylogenetic analysis indicates PAPT genes originated from retrotransposition events post-amphibian-amniote divergence.

Conclusions:

  • hPAPT is a testis-specific poly(A) polymerase with structural and genomic similarities to mPAPT.
  • The low sequence homology suggests divergent evolution despite conserved intronless structure.
  • PAPT genes likely evolved through retrotransposition, playing a conserved role in mammalian spermatogenesis.