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Preparation of oligonucleotides without aldehyde abasic sites
A H Krotz1, H Gaus, V T Ravikumar
1Isis Pharmaceuticals Inc., 2292 Faraday Avenue, 92008, Carlsbad, CA, USA. akrotz@isisph.com
Bioorganic & Medicinal Chemistry Letters
|July 19, 2001
Abstract:
High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites leading to significantly increased retention.