Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Full-length cDNAs: more than just reaching the ends.

M Das1, I Harvey, L L Chu

  • 1Department of Biochemistry, McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6.

Physiological Genomics
|July 19, 2001
PubMed
Summary

Developing high-quality complementary DNA (cDNA) resources is crucial for functional genomics. This review analyzes cDNA library generation methods to overcome bottlenecks in full-length cDNA characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term cost-effectiveness of screening for fracture risk in a UK primary care setting: the SCOOP study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2020
Same author

Screening for high hip fracture risk does not impact on falls risk: a post hoc analysis from the SCOOP study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2020
Same author

Systematic screening using FRAX<sup>®</sup> leads to increased use of, and adherence to, anti-osteoporosis medications: an analysis of the UK SCOOP trial.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2019
Same author

Sex differences in ventricular-vascular coupling following endurance training.

European journal of applied physiology·2014
Same author

CT angiography-guided single-stage release of adjacent webspaces in non-Apert syndactyly.

The Journal of hand surgery, European volume·2014
Same author

Assessment of point-of-care measurement of international normalised ratio using the CoaguChek XS Plus system in the setting of acute ischaemic stroke.

Internal medicine journal·2013

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Functional genomic resources are vital for interpreting genome sequencing data.
  • High-quality complementary DNA (cDNA) resources and advanced mRNA analysis technologies are key.
  • Isolating and mapping cDNAs aids in characterizing biologically relevant genomic information.

Purpose of the Study:

  • To review the current state-of-the-art in cDNA library generation.
  • To analyze the different steps involved in creating cDNA libraries.
  • To identify and discuss bottlenecks in parallel full-length cDNA development.

Main Methods:

  • Literature review of existing cDNA library generation techniques.
  • Analysis of iterative screening and directed strategies for cDNA processing.

Related Experiment Videos

  • Comparative assessment of various cDNA library quality and generation methods.

    • Main Results:

    • Identified parallel full-length cDNA development as a significant bottleneck.
    • Highlighted the time-consuming and labor-intensive nature of current methods.
    • Provided an overview of established and emerging cDNA library generation strategies.

    Conclusions:

    • Optimizing cDNA library generation is essential for advancing functional genomics.
    • Improved technologies are needed to streamline the characterization of coding and noncoding mRNA.
    • Efficient cDNA resources facilitate a deeper understanding of organismal genomes.