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Fibrinogen modulates gene expression in wounded fibroblasts
M Pereira1, P J Simpson-Haidaris
1Department of Medicine-Vascular Medicine Unit, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
Annals of the New York Academy of Sciences
|July 20, 2001
Summary
Fibrinogen (FBG) contributes to wound repair by assembling into the extracellular matrix (ECM) and modulating gene expression, suggesting roles beyond hemostasis.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Fibrinogen (FBG) is traditionally known for its hemostatic function.
- Recent findings indicate extrahepatic FBG synthesis and its presence in the extracellular matrix (ECM).
- Both plasma-derived and lung epithelial cell-derived FBG incorporate into the ECM of various cell types.
Purpose of the Study:
- To investigate the role of FBG in modulating gene expression during wound repair.
- To determine if FBG assembly into the ECM is dependent on new protein synthesis.
Main Methods:
- Utilized an in vitro wound repair model.
- Employed cDNA array analysis to assess gene expression changes.
- Examined FBG's role in ECM assembly independently of de novo protein synthesis.
Main Results:
- FBG assembly into the ECM is a cell-dependent process.
- FBG downregulates fibronectin mRNA levels.
- FBG upregulates cyclin D1 mRNA levels in fibroblasts.
Conclusions:
- FBG plays a role in cellular adhesive interactions and signaling during wound repair.
- FBG's functions extend beyond hemostasis, influencing gene expression.
- FBG modulates key genes involved in cellular processes during tissue repair.