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The mouse Mps1p-like kinase regulates centrosome duplication

H A Fisk1, M Winey

  • 1MCD Biology, UCB 347, University of Colorado, Boulder, Boulder, CO 80309, USA.

Cell
|July 20, 2001
PubMed

Insights

Mouse Mps1p (mMps1p) protein kinase controls centrosome duplication, working with Cdk2. mMps1p stability, regulated by Cdk2, is crucial for this process during S phase.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The yeast Mps1p protein kinase is essential for centrosome duplication and the spindle assembly checkpoint.
  • Understanding the function of Mps1p orthologs in mammalian cells is crucial for comprehending cell cycle regulation.

Purpose of the Study:

  • To investigate the role of the mouse Mps1p ortholog (mMps1p) in centrosome duplication.
  • To elucidate the regulatory relationship between mMps1p and Cdk2 in controlling centrosome duplication.

Main Methods:

  • Localization studies of endogenous and overexpressed mMps1p in mouse cells using immunofluorescence.
  • Analysis of centrosome duplication in response to mMps1p overexpression and kinase-deficient mMps1p mutants.
  • Investigating the effect of Cdk2 inhibition on mMps1p localization and centrosome duplication.

Main Results:

  • Endogenous and overexpressed mMps1p localize to centrosomes and kinetochores in mouse cells.
  • Overexpression of mMps1p leads to centrosome reduplication during S phase arrest, while a kinase-deficient mutant inhibits duplication.
  • Cdk2 is required for mMps1p-mediated centrosome reduplication, and Cdk2 inhibition destabilizes mMps1p at centrosomes.

Conclusions:

  • Mouse Mps1p (mMps1p) is a key regulator of centrosome duplication in mammalian cells.
  • Cdk2 regulates mMps1p stability during S phase, thereby controlling its function in centrosome duplication.
  • mMps1p functions in concert with Cdk2 to ensure proper centrosome duplication.

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