Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temporal translational control by a metastable RNA structure.

J Møller-Jensen1, T Franch, K Gerdes

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense University, DK-5230 Odense M, Denmark.

The Journal of Biological Chemistry
|July 20, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An anaerobic in vitro flow model for studying interactions at the gastrointestinal host-microbe interface.

NPJ biofilms and microbiomes·2025
Same author

Landscape review about the decision to euthanize a compromised pig.

Porcine health management·2024
Same author

A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.

Science (New York, N.Y.)·2012
Same author

Expression of heat-shock protein 90 in glucocorticoid-sensitive and -resistant childhood acute lymphoblastic leukaemia.

Leukemia·2003
Same author

A pH-jump approach for investigating secondary structure refolding kinetics in RNA.

Nucleic acids research·2002
Same author

Bacterial toxin RelE induces apoptosis in human cells.

FEBS letters·2002

The hok/sok locus uses a novel 5' metastable structure in hok mRNA to prevent premature translation and antisense RNA binding. This structure is essential for programmed cell death regulation in plasmid R1.

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • The hok/sok locus of plasmid R1 regulates programmed cell death through complex translational control.
  • Activation of hok mRNA involves 3'-end processing, triggering refolding and potential binding by Sok antisense RNA.
  • RNase III-dependent mechanisms inactivate hok mRNA upon Sok RNA binding.

Purpose of the Study:

  • To provide genetic evidence for a 5' metastable structure in hok mRNA.
  • To investigate the role of this structure in regulating translation and antisense RNA binding.
  • To elucidate the post-transcriptional control mechanisms of programmed cell death.

Main Methods:

  • Genetic analysis to identify and characterize the 5' metastable structure in hok mRNA.
  • In vitro assays to assess the impact of the metastable structure on Sok RNA binding and translation.

Related Experiment Videos

  • Structural analyses of native RNAs to confirm the existence and conformation of the 5' structure.

    • Main Results:

    • Genetic evidence confirms the in vivo existence of a 5' metastable structure in nascent hok mRNA.
    • This structure effectively blocks hok mRNA translation and reduces Sok RNA binding in vitro.
    • Structural analyses support the presence of the metastable structure and its refolding into a stable conformation upon 3' end processing.

    Conclusions:

    • A 5' metastable structure in hok mRNA acts as an intrinsic translational and antisense binding repressor.
    • Post-transcriptional 3'-end processing releases this structure, enabling translation and Sok RNA interaction.
    • This intricate mechanism provides profound insights into the regulation of programmed cell death.