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Updated: Aug 4, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
Retroviral splicing suppressor requires three nonconsensus uridines in a 5' splice site-like sequence
R E Paca1, C S Hibbert, C T O'Sullivan
1Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Abstract:
Rous sarcoma virus RNA contains a negative regulator of splicing (NRS) element that aids in maintenance of unspliced RNA. The NRS binds U1 snRNA at a sequence that deviates from the 5' splice site consensus by substitution of U's for A's at three positions: -2, +3, and +4. All three of these U's are important for NRS-mediated splicing suppression. Substitution of a single nonconsensus C or G at any of these sites diminished NRS activity, whereas substitution of a single A generated a preferred 5' splice site within the NRS.
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