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Isolation and characterization of two novel A20-like proteins
P C Evans1, E R Taylor, J Coadwell
1Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK. paul.evans@bbsrc.ac.uk
The Biochemical Journal
|July 21, 2001
Summary
Two novel genes, Cezanne and TRABID, were identified with sequence similarity to A20, a negative regulator of nuclear factor kappa B (NF-kappa B). Cezanne specifically down-regulates NF-kappa B, suggesting a role in inflammatory processes.
Area of Science:
- Molecular Biology
- Immunology
Background:
- Nuclear factor kappa B (NF-kappa B) is a key transcription factor in inflammatory responses.
- A20 is a zinc finger protein that negatively regulates NF-kappa B.
- The precise mechanism of A20's regulation and its interactions with signaling molecules are not fully understood.
Purpose of the Study:
- To identify novel genes with sequence similarity to A20.
- To investigate the interaction of these novel genes with TRAF molecules.
- To determine the role of these novel genes in the regulation of NF-kappa B.
Main Methods:
- Sequence analysis to identify genes similar to A20.
- Co-immunoprecipitation assays to study protein interactions.
- Reporter gene assays to assess NF-kappa B activity.
Main Results:
- Two novel genes, Cezanne and TRABID, were identified.
- Both Cezanne and TRABID were found to interact with TRAF6.
- Cezanne demonstrated a specific ability to down-regulate NF-kappa B activity.
Conclusions:
- Cezanne and TRABID are novel genes related to A20.
- Cezanne interacts with TRAF6 and specifically inhibits NF-kappa B.
- Cezanne likely plays a role in regulating inflammatory processes.