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Related Experiment Videos

Quantitation of gold lymphocyte binding during chrysotherapy.

A Lorber, S Wilcox, J Leeb

    The Journal of Rheumatology
    |May 1, 1979
    PubMed
    Summary

    Carbon rod atomic absorption analysis accurately quantifies gold lymphocyte content (GLC) in chrysotherapy. This method offers superior sensitivity and safety compared to traditional techniques, enabling new insights into gold

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    Area of Science:

    • Analytical Chemistry
    • Clinical Chemistry
    • Pharmacology

    Background:

    • Chrysotherapy involves administering gold compounds to treat inflammatory conditions like rheumatoid arthritis.
    • Accurate measurement of gold distribution within lymphocytes is crucial for understanding treatment efficacy and toxicity.
    • Existing methods for gold quantification have limitations in sensitivity, accuracy, and clinical applicability.

    Purpose of the Study:

    • To quantify gold lymphocyte content (GLC) during chrysotherapy using carbon rod atomic absorption analysis (CRA).
    • To compare the sensitivity and accuracy of CRA with 195Au isotopic scintillation counting.
    • To investigate the relationship between GLC, plasma gold levels, and cumulative dosage in patients undergoing chrysotherapy.

    Main Methods:

    • Utilized carbon rod atomic absorption analysis (CRA) for gold quantification.
    • Analyzed picogram levels of gold in limited sample volumes (less than 10 ml blood, specifically 5 microliters of lymphocytes).
    • Compared CRA results with 195Au isotopic scintillation counting.

    Main Results:

    • CRA demonstrated favorable sensitivity and accuracy compared to 195Au isotopic scintillation counting.
    • GLC significantly increased in 60% of patients after intramuscular (IM) gold administration.
    • For patients on oral auranofin, GLC approached levels seen with IM gold, despite lower plasma gold concentrations.

    Conclusions:

    • CRA is a sensitive and accurate method for quantifying gold lymphocyte content (GLC) in clinical settings.
    • GLC levels can be independent of plasma gold content or cumulative dosage in some patients.
    • The high sensitivity of CRA allows for the analysis of GLC under various therapeutic conditions, potentially elucidating gold's functional impact on lymphocytes.

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