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Related Experiment Videos

Responding phospholipid membranes--interplay between hydration and permeability.

E Sparr1, H Wennerström

  • 1Division of Physical Chemistry 1, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden. emma.sparr@fkem1.lu.se

Biophysical Journal
|July 21, 2001
PubMed
Summary

Osmotic pressure significantly impacts skin barrier function by altering lipid behavior and water transport. This study models how osmotic gradients influence molecular diffusion and phase transitions in lipid bilayers, explaining skin occlusion effects.

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Area of Science:

  • Biophysics
  • Physical Chemistry
  • Dermatology

Background:

  • Osmotic forces regulate physiological membrane processes.
  • Stratum corneum (SC) lipids are crucial for skin barrier function due to water potential gradients.
  • Understanding osmotic effects on SC lipid phase behavior is vital for skin physiology.

Purpose of the Study:

  • To develop a theoretical model for molecular diffusional transport across lipid bilayers under osmotic pressure gradients.
  • To investigate the impact of osmotic pressure on lipid phase behavior and its influence on diffusion.
  • To provide a physical basis for the skin occlusion effect.

Main Methods:

  • A theoretical model for molecular diffusional transport over a two-component lipid bilayer stack was developed.

Related Experiment Videos

  • The model incorporates interlamellar forces and regular solution theory to predict lipid phase behavior.
  • Calculations simulated water and nicotine flux through the bilayer stack under osmotic gradients.

    • Main Results:

    • Osmotic pressure gradients drive water diffusion and induce gradients in swelling and phase transformations.
    • A feedback mechanism between osmotic pressure and lipid phase behavior leads to nonlinear transport.
    • Calculated water flux qualitatively matched experimental SC water flux data.
    • A first-order phase transformation (gel to liquid-crystalline) was induced by increased osmotic pressure.

    Conclusions:

    • Osmotic pressure gradients significantly alter lipid bilayer properties and molecular diffusion.
    • The model explains the occlusion effect and provides insights into skin barrier regulation.
    • Water chemical potential boundary conditions can modulate membrane permeability, acting as a switch.