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DNA copy number alterations in HIV-positive and HIV-negative patients with diffuse large-cell lymphomas
M I Tiirikainen1, B P Mullaney, E A Holly
1Cancer Center, University of California at San Francisco, San Francisco, California, USA.
Journal of Acquired Immune Deficiency Syndromes (1999)
|July 21, 2001
Summary
HIV-positive individuals with diffuse large B-cell lymphoma (DLCL) show fewer genetic changes than HIV-negative patients. This suggests other factors, like immune surveillance, may drive DLCL progression in HIV-infected individuals.
Area of Science:
- Oncology
- Genetics
- Immunology
Background:
- Individuals with HIV have a higher risk of aggressive non-Hodgkin's lymphoma with a poorer prognosis.
- The genetic basis for differences in tumor behavior between HIV-positive and HIV-negative lymphoma patients is unknown.
Purpose of the Study:
- To investigate if lymphomas from HIV-positive individuals exhibit distinct somatic genetic alterations.
- To explore the genetic underpinnings of disease progression and outcome in HIV-associated lymphoma.
Main Methods:
- Genome-wide DNA copy number alterations (CNAs) were quantified in primary diffuse large B-cell lymphoma (DLCL) tumors.
- Comparative genomic hybridization (CGH) was used for 14 HIV-positive and 11 HIV-negative patients.
Main Results:
- HIV-positive DLCL tumors showed fewer regional DNA-CNAs and lower frequency of alterations compared to HIV-negative tumors.
- Frequent changes in HIV-negative DLCL included gains at chromosomes 8q and Xp.
- Gains on chromosomes 2p and 12q were common in both groups; no alteration was specific to AIDS-related DLCL.
Conclusions:
- Fewer somatic genomic changes may be required for DLCL progression in HIV-immunocompromised hosts.
- Reduced immune surveillance in HIV-infected individuals may contribute to lymphoma development and progression.