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Evidence of chromaffin oxygen sensing in neuroblastoma
F Hedborg1, G Franklin, J Norrman
1Department of Genetics and Pathology, University Hospital, Uppsala, Sweden. fredrik.hedborg@genpat.uu.se
Medical and Pediatric Oncology
|July 24, 2001
Abstract:
With the aid of IGF2 and VEGF in situ hybridization; tyrosine hydroxylase, chromogranin A, and Ki67 immunohistochemistry; and TUNEL staining applied to a large series of clinical neuroblastomas and to an animal model, we show here that stroma-poor neuroblastomas show evidence of chromaffin differentiation similar to that of type 1 small intensely fluorescent (SIF) cells and that this occurs in a vascular-dependent fashion, indicating a role for local tumor hypoxia in the differentiation process.