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PCR clamping

¹PNA Diagnostics A/S, Copenhagen, Denmark.

Current Issues in Molecular Biology

|July 24, 2001

Abstract:

An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.

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