Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human developing motor neurons as a tool to study ALS.

V Silani1, M Braga, A Botturi

  • 1Institute of Neurology, University of Milan Medical School, IRCCS Ospedale Maggiore, Italy. Vincenzo.Silani@venere.inet.it

Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders : Official Publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases
|July 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Array-comparative genomic hybridization analysis in a cohort of 130 children with Autism Spectrum Disorders: A single Center Italian Study.

La Clinica terapeutica·2023
Same author

Single-nucleotide polymorphisms in 3'-untranslated region inducible costimulator gene and the important roles of miRNA in alopecia areata.

Skin health and disease·2022
Same author

An innovative compact system for advanced treatment of combined sewer overflows (CSOs) discharged into large lakes: Pilot-scale validation.

Journal of environmental management·2019
Same author

Androgen insensitivity syndrome.

European review for medical and pharmacological sciences·2018
Same author

MRI and US in the evaluation of fetal anomalies: The need to work together.

Prenatal diagnosis·2017
Same author

Human Schwann Cell Proliferation and IL-6 Production following TNF-α Stimulation in Vitro.

Annals of the New York Academy of Sciences·2017

This study explores human motor neuron (hMN) vulnerability in amyotrophic lateral sclerosis (ALS) using cell culture models. Research identifies critical cellular events in ALS pathogenesis, aiding potential therapeutic development.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Genetics

Background:

  • Amyotrophic lateral sclerosis (ALS) pathogenesis is poorly understood, particularly the selective vulnerability of human motor neurons (hMNs).
  • Tissue culture models provide a platform to investigate hMN-specific features at the single-cell level.
  • Understanding hMN vulnerability is key to unlocking ALS disease mechanisms.

Purpose of the Study:

  • To define the basis of selective human motor neuron vulnerability in ALS.
  • To utilize in vitro models for identifying critical cellular events in ALS pathogenesis.
  • To facilitate the development of targeted ALS therapies.

Main Methods:

  • Isolation and culture of primary human motor neurons and astrocytes from embryonic spinal cords.

Related Experiment Videos

  • Single-cell analysis using cDNA/mRNA amplification techniques.
  • In vitro testing of molecular effects on hMN survival, neurite extension, and metabolism.
  • Gene expression analysis via DNA microarray technology.

    • Main Results:

    • Established methods for culturing and analyzing primary human motor neurons.
    • Demonstrated the utility of in vitro models for assessing molecular impacts on hMNs.
    • Identified potential targets for therapeutic intervention in ALS.

    Conclusions:

    • In vitro models of hMNs are crucial for understanding ALS pathogenesis and identifying therapeutic strategies.
    • This approach bypasses limitations of non-human models, offering direct clinical relevance.
    • Further research using these models can accelerate the development of effective ALS treatments.