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Related Experiment Videos

Development and maintenance of a B220- memory B cell compartment.

D J Driver1, L J McHeyzer-Williams, M Cool

  • 1Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|July 24, 2001
PubMed
Summary

A novel B220(-) memory B cell subset emerges after initial antigen exposure, not secondary challenge. These cells accumulate mutations and differentiate into plasma cells, representing a unique product of the primary immune response.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • A novel B220(-) memory B cell subset has been identified that dominates secondary immune responses.
  • The origin and development of this B220(-) memory B cell compartment remain unclear.

Purpose of the Study:

  • To investigate whether the B220(-) memory B cell compartment develops as a consequence of secondary antigen challenge or during the primary immune response.

Main Methods:

  • Adoptive transfer of B220(+)NP(+) memory B cells.
  • Analysis of B cell populations in the spleen at various time points after initial antigen exposure.
  • Cell surface phenotyping (GL7, BLA-1, CD24, CD43, CD11b) and in situ analysis.

Main Results:

  • B220(-)NP(+) B cells gradually emerge in the spleen, reaching maximal numbers 3 weeks after primary antigen exposure.

Related Experiment Videos

  • Initially unmutated, B220(-) B cells accumulate affinity-increasing mutations between days 9-14 of the primary response.
  • Phenotypic analysis suggests these cells are not localized in germinal centers but are found in the spleen's red pulp.

    • Conclusions:

    • B220(-) memory B cells develop as a unique cellular consequence of primary antigen exposure.
    • These cells are distinct products of the germinal center reaction and are maintained long-term in the spleen and bone marrow.