Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subunit interactions and AMPA receptor desensitization.

A Robert1, S N Irizarry, T E Hughes

  • 1Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520-8066, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|July 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antioxidant status and oxidative stress in professional rugby players: evolution throughout a season.

International journal of sports medicine·2006
Same author

Bulk milk somatic cell counts and bovine viral diarrhoea virus (BVDV) infection in 7252 dairy herds in Brittany (western France).

Preventive veterinary medicine·2005
Same author

Genetic abnormalities associated with the t(12;21) and their impact in the outcome of 56 patients with B-precursor acute lymphoblastic leukemia.

Cancer genetics and cytogenetics·2005
Same author

Value of intravenous 6-mercaptopurine during continuation treatment in childhood acute lymphoblastic leukemia and non-Hodgkin's lymphoma: final results of a randomized phase III trial (58881) of the EORTC CLG.

Leukemia·2005
Same author

Multidetector ct diagnosis of jejunal perforation by a chicken bone.

JBR-BTR : organe de la Societe royale belge de radiologie (SRBR) = orgaan van de Koninklijke Belgische Vereniging voor Radiologie (KBVR)·2005
Same author

A new recurrent inversion, inv(7)(p15q34), leads to transcriptional activation of HOXA10 and HOXA11 in a subset of T-cell acute lymphoblastic leukemias.

Leukemia·2005

This study reveals how AMPA receptor desensitization is modulated by subunit composition. Co-expressing wild-type and mutant subunits reveals tetrameric assemblies, impacting glutamate receptor function.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • AMPA-type glutamate receptors (GluRs) are crucial for synaptic transmission.
  • Receptor desensitization significantly influences excitatory postsynaptic current (EPSC) decay.
  • Mechanisms of AMPA receptor desensitization remain incompletely understood.

Purpose of the Study:

  • To investigate the role of subunit composition in AMPA receptor desensitization.
  • To elucidate the conformational changes underlying glutamate receptor desensitization.
  • To determine the stoichiometry of AMPA receptor assemblies.

Main Methods:

  • Co-expression of wild-type GluR1 and a desensitization-impaired mutant (GluR1(L497Y)).
  • Electrophysiological recordings of glutamate-evoked currents.

Related Experiment Videos

  • Analysis of desensitization kinetics and decay components.

    • Main Results:

    • Heteromeric channels formed by wild-type and mutant GluR1 subunits exhibit intermediate desensitization.
    • Desensitization kinetics correlate with the relative abundance of wild-type and mutant subunits.
    • Subunit distribution into tetrameric assemblies, not pentameric, explains observed desensitization patterns.

    Conclusions:

    • AMPA receptors likely function as tetramers, composed of two dimers.
    • Subunit composition critically regulates AMPA receptor desensitization.
    • Findings align with recent structural models of AMPA receptor quaternary structure.