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Related Experiment Videos

Measurement of insecticide uptake and effective fraction in a beneficial insect using solid-phase microextraction.

A Alix1, D Collot, J P Nénon

  • 1Laboratoire de Toxicologie Analytique, Faculté de Pharmacie, Rennes, France. alix@versailles.inra.fr

Analytical Chemistry
|July 27, 2001
PubMed
Summary

This study introduces a sensitive solid-phase microextraction (SPME) method to measure organophosphorus insecticide uptake in beneficial insects. The technique accurately quantifies low insecticide doses, aiding toxicological risk assessment in agricultural fields.

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Area of Science:

  • Environmental Chemistry
  • Toxicology
  • Entomology

Background:

  • Quantifying insecticide uptake in beneficial insects is crucial for assessing toxicological effects and field exposure.
  • The small size of beneficial insects poses challenges for measuring absorbed insecticide doses.

Purpose of the Study:

  • To present a sensitive method for measuring very small amounts of organophosphorus insecticides in small beneficial insects.
  • To apply this method to quantify internal doses and free dissolved fractions of chlorfenvinphos in parasitoids.
  • To determine insecticide penetration and elimination rates in insects.

Main Methods:

  • Utilized solid-phase microextraction (SPME) for its sensitivity and specificity in sampling.
  • Applied the SPME method to quantify chlorfenvinphos in beneficial parasitoids after topical application.

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  • Determined insecticide penetration and elimination rates.

    • Main Results:

    • The SPME method successfully quantified up to 0.5 ng of chlorfenvinphos, a 10-fold improvement over solvent extraction.
    • The method was effective for quantifying internal doses in parasitoids from treated fields.
    • Penetration and elimination rates of the insecticide were successfully determined.

    Conclusions:

    • Solid-phase microextraction (SPME) is a suitable technique for measuring low internal insecticide doses in small beneficial insects.
    • This method enhances the accuracy of toxicological risk assessment for beneficial insects exposed to insecticides in agricultural settings.
    • The developed method aids in understanding insecticide pharmacokinetics in non-target organisms.