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Immobilized fluorescent cyclodextrin on a cellulose membrane as a chemosensor for molecule detection
T Tanabe1, K Touma, K Hamasaki
1Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Analytical Chemistry
|July 27, 2001
Summary
A novel fluorescent sensor was developed using dansylglycine-modified cyclodextrin immobilized on a cellulose membrane. This sensor detects molecules by changes in fluorescence intensity, proving cellulose membranes are practical for modified cyclodextrins.
Area of Science:
- Supramolecular Chemistry
- Analytical Chemistry
- Materials Science
Background:
- Cyclodextrins are widely used as host molecules in various applications.
- Fluorescent probes offer sensitive detection methods for analytes.
- Immobilization of molecular probes onto solid supports enhances their usability.
Purpose of the Study:
- To develop a novel fluorescent chemosensor based on modified cyclodextrin.
- To investigate the immobilization of dansylglycine-modified cyclodextrin (DnsC4-beta-CD) onto a cellulose membrane.
- To evaluate the sensing capabilities of the DnsC4-beta-CD membrane for guest molecules.
Main Methods:
- Synthesis of dansylglycine-modified cyclodextrin (DnsC4-beta-CD).
- Immobilization of DnsC4-beta-CD onto a cellulose membrane.
- Fluorescence spectroscopy to monitor changes upon guest molecule binding.
Main Results:
- The DnsC4-beta-CD membrane exhibited decreased fluorescence intensity with increasing guest molecule concentration.
- The fluorescence quenching mechanism indicated a conformational change of the dansyl group upon guest accommodation.
- The cellulose membrane proved to be a suitable support for the functionalized cyclodextrin.
Conclusions:
- The DnsC4-beta-CD membrane functions as an effective chemosensor for molecule detection.
- Cellulose membranes are practical and versatile supporting materials for chromophore-modified cyclodextrins.
- This approach offers a novel platform for developing sensitive and reusable molecular sensors.