Microencapsulation of beta-galactosidase with fatty acid esters

The present study was carried out to find an appropriate coating material to microencapsulate lactase and the conditions under which it could be used. As a coating material, medium-chain triacylglycerol (MCT) and polyglycerol monostearate (PGMS) were superior. Three different percentages of microcapsules (2, 4, and 6%) were added and subdivided into three groups by washing time (0, 1, and 2). The highest efficiency of microencapsulation was found in the ratio of 15:1 as coating to core material with both MCT (94.9%) and PGMS (72.8%). Lactose content was not significantly changed up to 12 d with MCT and up to 8 d with PGMS with 2% of twice washed microcapsule addition. Also, sweetness was not significantly increased in storage compared with that of market milk. The present study provides evidence that emulsifiers can be used as an effective coating material to microencapsulate lactase. In addition, these results suggest that acceptable milk products can be prepared with microencapsulated lactase.