Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

COMO: a program for combined molecular replacement.

G Jogl1, X Tao, Y Xu

  • 1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.

Acta Crystallographica. Section D, Biological Crystallography
|July 27, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HED-Derived iPSCs Reveal Neurofunctional Defects in Ectodermal Dysplasia.

Journal of dental research·2026
Same author

[Microsatellite status and minimal microsatellite shift in atypical endometrial hyperplasia and endometrial cancer: an analysis of 848 cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

Janus AsP monolayers: a promising 2D platform for NO and NO<sub>2</sub> gas sensing.

Physical chemistry chemical physics : PCCP·2026
Same author

[A review of massive perivillous fibrin deposition in the placenta].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Massive perivillous fibrin deposition in twin placentas: a clinicopathological analysis of eleven cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Atypical forms of microglandular hyperplasia of the cervix: a clinicopathological analysis of 29 cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

A new computational method, COMO, integrates rotational and translational searches for molecular replacement, streamlining structure determination. This approach enhances efficiency and successfully solves complex crystallographic problems, including those with high symmetry.

Area of Science:

  • Structural Biology
  • Computational Crystallography
  • Biophysics

Background:

  • Molecular replacement is a key method for solving crystal structures.
  • Traditional methods often require separate optimization of rotational and translational parameters.
  • Complex structures and high-symmetry cases present significant challenges for molecular replacement.

Purpose of the Study:

  • To introduce a novel combined molecular replacement protocol.
  • To present the COMO computer program implementing this protocol.
  • To demonstrate the program's capability in solving difficult molecular replacement problems.

Main Methods:

  • A six-dimensional search combining rotational and translational parameter sampling.
  • Rotation function guides the sampling of rotational degrees of freedom.

Related Experiment Videos

  • Optimized Patterson correlation translation function calculation and implemented packing check.

    • Main Results:

    • The COMO program successfully integrates rotational and translational searches.
    • Optimized calculations and packing checks improve speed and signal.
    • Demonstrated ability to solve complex problems, including orienting five search model copies in a high-symmetry space group.

    Conclusions:

    • The combined molecular replacement protocol offers an efficient approach to structure solution.
    • COMO provides an automated and user-friendly tool for crystallographic structure determination.
    • The method is effective for challenging molecular replacement scenarios.