Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase amplification and detection: a single-tube diagnostic assay for infectious agents.

M Somodevilla-Torres1, P Timms, R Harris

  • 1Cooperative Research Centre for Diagnostic Technologies, Queensland University of Technology, Brisbane, Qld 4001, Australia.

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
|July 27, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology of Chlamydia psittaci infections in pregnant Thoroughbred mares and foals.

Veterinary journal (London, England : 1997)·2021
Same author

Antibody response against koala retrovirus (KoRV) in koalas harboring KoRV-A in the presence or absence of KoRV-B.

Scientific reports·2019
Same author

Induction of neutralizing antibody response against koala retrovirus (KoRV) and reduction in viral load in koalas following vaccination with recombinant KoRV envelope protein.

NPJ vaccines·2018
Same author

Genomewide DNA methylation analysis in combat veterans reveals a novel locus for PTSD.

Acta psychiatrica Scandinavica·2017
Same author

Sero-epidemiological assessment of Chlamydia trachomatis infection and sub-fertility in Samoan women.

BMC infectious diseases·2016
Same author

Brain-specific epigenetic markers of schizophrenia.

Translational psychiatry·2015

A new assay, sequential nucleic acid amplification and capture (SNAAC), offers a simple and rapid method for detecting infectious agents. This technique is cost-effective and adaptable for clinical labs.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Development of a novel single-tube assay for infectious agent detection.
  • Assay designed in a microtiter tray format, similar to ELISA.
  • Focus on improving diagnostic capabilities for pathogens.

Purpose of the Study:

  • To introduce and validate the Sequential Nucleic Acid Amplification and Capture (SNAAC) method.
  • To demonstrate the utility of SNAAC for detecting specific infectious agents.
  • To assess the adaptability of SNAAC for clinical laboratory use.

Main Methods:

  • Sequential Nucleic Acid Amplification and Capture (SNAAC) combines amplification and hybridization.
  • Target amplification using species-specific primers with detection tags (e.g., fluorescein, biotin).

Related Experiment Videos

  • Hybridization of amplified product to surface-bound oligonucleotide probes in microtiter wells, followed by colorimetric detection.

    • Main Results:

    • Successfully demonstrated the SNAAC method using cloned sequences of Chlamydia pneumoniae.
    • Validated the capture and detection of amplified nucleic acid sequences.
    • Showcased the ability to detect low levels of target pathogens.

    Conclusions:

    • SNAAC is a simple, rapid, and inexpensive diagnostic method.
    • The assay is readily adaptable to existing clinical laboratory equipment.
    • Avoids the need for specialized or new instrumentation for infectious agent detection.