Related Experiment Video
Updated: Aug 9, 2026
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
Perfusion of human ovaries in vitro with artificial medium: metabolism and steroidogenesis
American Journal of Obstetrics and Gynecology
|August 1, 1975
Abstract:
A study was made of ovaries perfused in vitro with a liquid fluorocarbon that was stabilized in emulsion by a nonionic detergent to which tissue culture medium was added. A pulsatile flow was used in an organ preservation system under aseptic conditions. Glucose was utilized and lactate produced at a fairly constant rate for as long as 20 hours of perfusion. 14-C-progesterone and 14-C-17alphaOH-progesterone were synthesized from 14-C-acetate and estradiol was secreted. The production of these steroids by the perfused ovary appears to be responsive to gonadotropins.
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