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Updated: Aug 7, 2026
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Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
Published on: June 8, 2019
Effects of ischemia on gene expression
1Department of Surgery and Biostatistics, H. Lee Moffitt Cancer Center, University of South Florida, 12902 Magnolia Drive, Tampa, FL 33647, USA.
The Journal of Surgical Research
|July 27, 2001
Summary
Warm ischemia significantly alters gene expression in human tissues. Monitoring ischemic time is crucial for accurate gene profiling in surgical samples.
Area of Science:
- Molecular Biology
- Genomics
- Surgical Pathology
Background:
- Microarray technology enables large-scale RNA expression analysis.
- Surgical procedures often involve warm ischemia, potentially affecting gene expression profiles.
Purpose of the Study:
- To quantify the impact of warm ischemia on human tissue gene expression.
- To identify patterns of gene expression changes induced by varying ischemic times.
Main Methods:
- Normal colon mucosa was dissected and snap-frozen at multiple time points (5-60 min) after extirpation.
- Microarrays with 2400 elements assessed mRNA from each time point in triplicate.
- Eisen's hierarchical clustering and Bayesian methods analyzed gene expression changes over time.
Main Results:
- Three distinct gene expression patterns emerged, influenced by ischemia duration.
- Pattern I showed an average 27% increase in expression for 63.8% of genes over 60 min.
- Pattern III exhibited the greatest sensitivity, with a 50% average change in expression, including significant fold changes up to 20-fold.
Conclusions:
- Warm ischemia significantly impacts human tissue gene expression profiles.
- Gene expression changes vary, with some patterns showing increases and others decreases.
- Careful monitoring of ischemic time is essential for reliable gene profiling in harvested tissues.
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