Related Experiment Videos
Tract microflora in Saudi patients with cholelithiasis
M Al Harbi1, A O Osoba, A Mowallad
1Department of Surgery, King Abdulaziz University Hospital, Jeddah, Saudi Arabia. malharbi@drcom
Tropical Medicine & International Health : TM & IH
|July 27, 2001
Summary
Gallbladder microflora in gallstone patients undergoing surgery was identified. Antibiotic susceptibility patterns and Gram staining usefulness were assessed, suggesting a review of current antibiotic prophylaxis for cholelithiasis management.
Area of Science:
- Microbiology
- Surgical Infectious Diseases
Background:
- Gallstones are a common condition requiring surgical intervention.
- Understanding gallbladder microflora is crucial for preventing surgical site infections.
Purpose of the Study:
- To identify gallbladder microflora in patients with gallstones undergoing laparoscopic cholecystectomy.
- To determine the antibiotic susceptibility of isolated microorganisms.
- To evaluate the utility of Gram staining for rapid bile analysis.
Main Methods:
- Bile samples from 112 patients were cultured for aerobic and anaerobic bacteria.
- Gram staining was performed on centrifuged bile deposits.
- Antibiotic susceptibility testing utilized the Kirby Bauer technique.
Main Results:
- 25% of bile samples yielded positive cultures, with Escherichia coli being the most common isolate.
- Gram staining correctly identified organisms in 67.8% of positive samples.
- Positive cultures were significantly associated with patients over 50, post-operative fever, and leucocytosis.
Conclusions:
- Age over 50 is a significant predictor of positive bile cultures.
- Current antibiotic prophylaxis guidelines for laparoscopic cholecystectomy may require revision.
- Further research into the role of bacteribilia in cholelithiasis management is warranted.