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Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Study of mycoloyl transferase transport across the cell envelope of Corynebacterium glutamicum

D T Nguyen1, C Houssin, N Bayan

  • 1Laboratoire des Biomembranes, Bât. 430, CNRS UMR 8619, Université de Paris XI, 15 rue Georges Clémenceau, 91405 Cedex, Orsay, France

FEMS Microbiology Letters
|July 27, 2001
PubMed

Insights

The study reveals Corynebacterium glutamicum

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • PS1 is a major exported protein in Corynebacterium glutamicum, homologous to mycobacterial antigen 85.
  • PS1 is associated with the mycolic acid-containing cell wall and functions as a mycoloyl transferase.

Purpose of the Study:

  • To investigate the transport mechanism of PS1 protein to the cell wall in Corynebacterium glutamicum.
  • To elucidate the distinct energetic requirements and kinetics of PS1 transport steps.

Main Methods:

  • Analysis of protein transport kinetics.
  • Inhibition studies using sodium azide and carbonyl cyanide m-chlorophenylhydrazone.
  • Investigation of ATP and proton motive force dependency.

Main Results:

  • PS1 transport occurs in two energetically distinct steps.
  • The first rapid step involves signal peptidase processing and is inhibited by azide or CCCP, suggesting cytoplasmic membrane translocation.
  • The second step's kinetics depend on polypeptide size and do not require ATP or proton motive force, indicating diffusion across the cell wall.

Conclusions:

  • The transport of PS1 protein to the cell wall is a complex, multi-step process.
  • The findings provide insights into the secretion pathway of cell wall-associated proteins in Gram-positive bacteria.

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