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Related Experiment Videos

Evidence for alternate splicing within the mRNA transcript encoding the DNA damage response kinase ATR.

J L Mannino1, W Kim, M Wernick

  • 1Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.

Gene
|July 27, 2001
PubMed
Summary

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Researchers discovered alternate splicing in the ATR gene

Area of Science:

  • Molecular Biology
  • Genetics
  • Cellular Biology

Background:

  • Cellular responses to DNA damage involve PIK-like kinases.
  • Understanding regulators of these kinases, particularly their non-catalytic domains, is crucial.
  • Limited knowledge exists regarding factors controlling PIK-like protein activity.

Purpose of the Study:

  • To investigate functional subdomains within the non-catalytic domain of the PIK-like protein ATR.
  • To determine if the ATR gene transcript undergoes alternative splicing.
  • To explore the functional significance of alternative splicing in ATR.

Main Methods:

  • Sequencing of human expressed sequence tag (EST) clones for ATR cDNA.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) on human RNA.

Related Experiment Videos

  • Cloning and sequencing of human genomic DNA segments.
  • Analysis of alternative ATR transcript expression in human tissues.

    • Main Results:

    • Identified alternative splicing in the mRNA region encoding the non-catalytic domain of ATR.
    • Confirmed alternative splicing arises from a single 192 bp exon in the ATR gene.
    • Demonstrated differential expression of alternative ATR transcripts across various human tissues.

    Conclusions:

    • The non-catalytic domain of ATR is subject to alternative splicing.
    • Alternative splicing of ATR transcripts suggests functional importance of this region.
    • Further research is warranted to elucidate the functional implications of ATR alternative splicing.