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[Human Fab antibodies derived from phage display library neutralize hepatitis A virus in vitro]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Objective:
Development of recombinant human monoclonal antibody to hepatitis A virus as a emergent measure for prevention of hepatitis A virus infection.
Methods:
Human neutralizing monoclonal antibody Fab fragments to HAV have been developed by using phage display technique. The heavy and light chains of human IgG Fab genes were amplified from a HAV patient in convalescent stage. The combinatorial phage antibody library was established by inserting both heavy and light chains of Fab genes into phage mid-vector pComb3 and followed by help phage infection after 4 rounds of panning with purified HAV as coated antigen.
Results:
The human Fab fragments to HAV were selected and expressed in bacteria.
Conclusions:
The specific binding of Fab antibodies to HAV were demonstrated by their reaction with HAV antigen in ELISA. These results provide the basis for further development of a neutralizing recombinant human whole IgG molecule and hold promise for future use in the prophylaxis of HAV infection.