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Production and Titering of Recombinant Adeno-associated Viral Vectors
Published on: November 27, 2011
[Construction and expression of recombinant adeno-associated HIV -1 virus]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Abstract
Objective:
Plasmid of AAV vector with HIV-1 gag and gp 120 were constructed respectively.
Methods:
The recombinant AAV-HIV gag and gp120 viruses were obtained by co transfection of AAV-HIV plasmid and Adenovirus 5 in 293 cells.
Results:
The recombinant AAV-HIV virus expressed well and the titer of the recombinant viruses were between 10(4)-10(5).
Conclusions:
The recombinant AAV-HIV virus can be used to infect the target cells for detection of cellular immunity and for vaccine studies.
