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Related Experiment Videos

In situ NMR systems.

J V Shanks1

  • 1Dept of Chemical Engineering, Iowa State University, Ames 50011-2230, USA.

Current Issues in Molecular Biology
|July 27, 2001
PubMed
Summary
This summary is machine-generated.

In situ Nuclear Magnetic Resonance (NMR) is a powerful tool for bioprocessing and metabolic engineering. This noninvasive biosensor provides real-time data for modeling cellular functions, enhancing bioprocess optimization.

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Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Analytical Chemistry

Background:

  • In situ Nuclear Magnetic Resonance (NMR) is emerging as a key technology in bioprocessing and metabolic engineering.
  • The in situ NMR biosensor functions as a noninvasive meter for pH, ions, and concentrations, primarily utilizing phosphorus-31 (31P) and carbon-13 (13C) isotopes.
  • Extensive spectral databases for phosphorus and carbon in bacteria and yeast are now available.

Purpose of the Study:

  • To highlight the capabilities of in situ NMR for providing essential state variables for modeling glycolytic pathway function.
  • To discuss advancements in NMR micro-reactor technology for bioprocess monitoring.
  • To emphasize the feasibility of studying aerobic, chemostat cultures using in situ NMR.

Main Methods:

Related Experiment Videos

  • Utilizing in situ NMR spectroscopy with 31P and 13C isotopes.
  • Employing NMR micro-reactor technology, including various designs for immobilized cell reactors.
  • Conducting studies on aerobic, chemostat cultures and cell suspensions (3-5% v/v).

    • Main Results:

    • In situ NMR provides crucial state variables for metabolic pathway modeling.
    • Significant improvements in NMR micro-reactor technology have been achieved.
    • Feasible application of in situ NMR for aerobic, chemostat cultures has been demonstrated.
    • 31P spectra acquisition from cell suspensions is possible with 3-7 minute time resolution.

    Conclusions:

    • In situ NMR is a valuable, noninvasive technology for real-time monitoring in bioprocessing and metabolic engineering.
    • Advancements in NMR technology enhance the study of cellular metabolism and bioprocesses.
    • The technique offers high time resolution for spectral acquisition, facilitating dynamic studies.