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NMR evidence for Mg(II) binding to N1 of ATP
1Laboratory of NMR and Atomic Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan, People's Republic of China.
Abstract:
The conformation of the complex of [ATP-Mg]2+ is studied by 1H, 15N and 31P NMR on ATP in the absence and presence of MgCl2 in a wide pH range from 1 to 10. 1H-15N HMBC experiments show a large change in the 15N chemical shift of N1 up to 10 ppm around pH 3.7, suggesting that there is a strong interaction between Mg2+ and N1 of ATP at this pH. 31P NMR indicates that at pH 3.7 the phosphate chain also binds Mg2+. 1H diffusion measurements imply that the [ATP-Mg]2+ complex involves only one ligand and one metal ion.