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Rapid method for the estimation of octanol/water partition coefficient (log P(oct)) from gradient RP-HPLC retention
1Hit Generation Sciences and Analytical Technologies, GlaxoWellcome Medicines Research Centre, Stevenage, Herts, SG1 2NY, United Kingdom.
Current Medicinal Chemistry
|July 27, 2001
Abstract:
We propose a rapid method for the measurement of octanol/water partition coefficients (log P(oct)) via fast gradient reversed phase retention and the calculation of the hydrogen bond acidity of the compounds. The cycle time of the generic gradient HPLC method is 5 minutes. The general solvation equation obtained for the log Poct values and the fast gradient Chromatographic Hydrophobicity Indices with acetonitrile (CHI(ACN)) and methanol