Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct B/B-cell interactions in immunoglobulin synthesis.

K Shinozaki1, K Yasui, K Agematsu

  • 1Department of Paediatrics, Graduate School of Medicine, Shinshu University, Matsumoto, Japan.

Clinical and Experimental Immunology
|July 27, 2001
PubMed
Summary

Direct B cell interactions via CD27/CD70 significantly boost immunoglobulin G (IgG) production and plasma cell differentiation. This study highlights a novel mechanism in B cell communication crucial for immune responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk factors for zolbetuximab-associated nausea and vomiting: a pharmacovigilance analysis using the Japanese Adverse Drug Event Report (JADER) database.

Die Pharmazie·2026
Same author

Factors predicting neurosensory disturbance after bilateral sagittal split ramus osteotomy: a retrospective cohort study.

International journal of oral and maxillofacial surgery·2024
Same author

Analysis and evaluation of factors contributing to the occurrence of immune-related adverse events with immune checkpoint inhibitors.

Die Pharmazie·2024
Same author

Dataset of night-time emissions of the Earth in the near UV range (290-430 nm), with 6.3 km resolution in the latitude range -51.6<L<+51.6 degrees, acquired on board the International Space Station with the Mini-EUSO detector.

Data in brief·2023
Same author

A Review of the EUSO-Balloon Pathfinder for the JEM-EUSO Program.

Space science reviews·2022
Same author

Large-scale prospective genome-wide association study of oxaliplatin in stage II/III colon cancer and neuropathy.

Annals of oncology : official journal of the European Society for Medical Oncology·2021

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The precise roles of B cell-to-B cell interactions in immune responses remain unclear.
  • Understanding these interactions is vital for elucidating humoral immunity mechanisms.

Purpose of the Study:

  • To investigate B cell-to-B cell interactions in immunoglobulin synthesis.
  • To explore the involvement of the tumor necrosis factor receptor (TNFR)/tumor necrosis factor (TNF) superfamily in these interactions.

Main Methods:

  • Purified human peripheral blood B cells were stimulated with Epstein-Barr virus (EBV)-transformed B lymphoblastoid cell lines (LCLs).
  • Immunoglobulin G (IgG) production was measured using flow cytometry and ELISA.
  • Expression of key surface molecules (CD40, CD70, CD154, CD27, OX40, CD134 ligand) was analyzed.

Related Experiment Videos

  • Blocking monoclonal antibodies (MoAbs) were used to investigate specific molecular interactions.

    • Main Results:

    • LCLs significantly enhanced IgG production by B cells in the presence of IL-10 and IL-2.
    • LCLs expressed high levels of CD40 and CD70.
    • The enhancement of IgG production was blocked by anti-CD70 MoAb, indicating CD70's critical role.
    • B cell CD27 expression was reduced by LCLs, an effect blocked by anti-CD70 MoAb, confirming CD27/CD70 interaction.
    • LCLs promoted B cell differentiation into plasma cells.

    Conclusions:

    • Direct B cell-to-B cell contact, specifically via the CD27/CD70 pathway, is a key mechanism for inducing immunoglobulin production.
    • This interaction promotes B cell differentiation into plasma cells, contributing significantly to humoral immune responses.